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4.1. Materials and methods
RuCl3·nH2O (J&K), bpy (J&K), phen (J&K), DIP (J&K), 5,6-diamino-1,10-phenanthroline (Alfa Aesar), 1,10-phenanthroline-5,6-dione (Alfa Aesar), Re(CO)5Cl (Sigma Aldrich), NH4PF6 (Alfa Aesar), DPBF (Alfa Aesar), MB (Alfa Aesar), Dulbecco's Modified Eagle Medium (DMEM, Gibco), Roswell Park Memorial Institute 1640 (RPMI 1640, Gibco), fetal bovine serum (Gibco), penicillin-streptomycin (Gibco), MTT (J&K), JC-1 Detection Kit (Beyotime), H2DCFDA (J&K), Hoechst 33342 (J&K), Annexin V-FITC Apoptosis Detection Kit (Beyotime), Matrigengel (ABW). Primary antibodies against caspase-3, PARP, MMP-2, MMP-9 and VEGFR2 were purchased from Cell Signaling Technology. RuRe1–3 were dissolved in DMSO (1%, v/v) just before the biological experiments.
IF:4.336
原文链接:https://doi.org/10.1016/j.jinorgbio.2022.112090(需复制到网页查看)
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