TIAM2对奥斯替尼的抵抗、细胞运动和M2样极化

自ABW&Mogengel基质胶推出以来，在业界赢得了良好的口碑，感谢广大客户们的认可！公司将一如既往严格把控产品质量，誓为广大科研工作者提供优质的产品和技术服务！

同时为了感谢和回馈广大的科研工作者，ABW&MG发起【引用ABW&MG基质胶，领取高额奖金】的长期有效活动，若您使用了ABW&MG产品并发表了文章，请联系我们领取相应的奖励哦！

4.12. Transwell Assay

Transwell assays were performed using transwell chambers with or without Matrigel coating to detect cell invasion and migration ability, respectively. For invasion assay, Matrigel (082706, ABW, China) was dissolved with a serum-free medium at 1:8, and 100 µL was added to each upper chamber. Then, 200 µL serum-free medium containing 5 × 104 was added to the upper chamber, while the lower chamber was added with 600 µL 10% FBS medium. The cells were fixed and stained after 24 h of incubation. For migration assay, 200 µL serum-free medium containing 2.5 × 104 was added to the upper chamber, while the lower chamber was added with 800 µL 10% FBS medium. The next procedures are the same as for the invasion assay. Three randomly selected fields of view were photographed under the microscope and the images were counted using ImageJ software (National Institutes of Health).

以下为引用欧洲杯买球官方网站（ABW）基质胶文献